|
KMID : 0545120020120030526
|
|
Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.526 ~ p.529
|
|
|
Hemolytic Activity of Culture Supernatant of Xenorhabdus nematophilus, a Symbiotic Bacterium of Entomopathogenic Nematodes
|
|
RYU, KEUN-GARP
BAE, JUN-SANG/Kwack, Kyu Bum/Kwon, O. Yul/Park, Sun Ho
|
|
|
Abstract
|
|
|
|
Lysis of erythrocytes isolated from human, rabbit, and mouse blood samples was investigated with the culture supernatant of Xenorhabdus nematophilus in a primary form. Prior to use, the culture supernatant of the bacteria was concentrated and the concentrate was dialyzed against Tris-HCl buffer (10mM, pH 8.1) by ultrafiltration using PM-5 membrane with a molecular weight cut-off of 5,000. At 30℃, the supernatant exhibited no lytic activity towards three types of erythrocytes. However, at 4℃, the supernatant showed selective lytic activity towards rabbit erythrocytes within 90 min, yet did not lyze human or mouse erythrocytes. Microscopic examination clearly revealed that most of the rabbit erythrocytes had been turned into ghost forms.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|